Critical control point design of negative pressure biosafety level-2 laboratories

With two examples of negative pressure biosafety level-2 laboratories, discusses the design of functional zoning and layout, ventilation and air conditioning modes, air distribution, air supply and exhaust systems. Offers following suggestions: to divide the core working room (or animal room) into the relatively clean side and the relatively polluted side, to arrange the buffer, dressing and preparation rooms, to use the all-air direct flow air conditioning system, to set the flow direction of the overall airflow from the clean area to the core working room (or animal room), and the airflow in the working room (or animal room) from the relatively clean side to the polluted side, to use the primary-intermediate-high efficiency three-stage filtration in the air supply system, and the one-stage high efficiency filtration in the air exhaust system, to design separate air exhaust in the biological safety cabinets, to incorporate the IVC and negative pressure cabinet exhaust as part of the laboratory exhaust system, and to set spare exhaust fans.